VEGF dynamical changes in laboratory rodents with transplanted experimental tumors of various histological types

Alexander P. Trashkov; Nikolay A. Verlov; Margarita R. Artemenko; Valeria A. Pechatnikova; Maria A. Zelenenko; Mariya A. Pakhomova; Andrei G. Vasiliev

VEGF dynamical changes in laboratory rodents with transplanted experimental tumors of various histological types

Alexander P. Trashkov Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”
Nikolay A. Verlov Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”
Margarita R. Artemenko Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”
Valeria A. Pechatnikova Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”
Maria A. Zelenenko Sechenov Institute of Ephysiology and Biochemistry, Russian Academy of Sciences
Mariya A. Pakhomova St. Petersburg State Pediatric Medical University
Andrei G. Vasiliev St. Petersburg State Pediatric Medical University

Abstract

Vascular endothelial growth factor (VEGF) is one of the most important cytokines in charge of proliferation, migration and differentiation of endothelial cells in physiological and pathological processes. Because of this they are involved in pathogenesis of neoplasmatic process namely mechanisms of neoangiogenesis – development of blood-vessels’ network in the tumor as well as adjoining intact tissues. Almost all contemporary antiangiogenic medicines are targeted at either VEGF or its receptors. However, there are practically no conventional models of oncologic pathology nowadays described in detail and recommended for preclinical studies. The present study focuses at changes of VEGF concentrations at va rious stages of disease using experimental tumors of different histological types, intensity of neoplasmatic growth and localization. Development of experimental transplantable tumors of various histological types and locations has been demonstrated to be usually accompanied by increased VEGF blood serum concentration in experimental animals; the dynamic of this increase depending upon the intensity of the tumor growth. A statistically valid decrease of VEGF level in comparison with the previous control point of the study has been demonstrated in BALB/c male mice with subcutaneously transplanted colonic adenocarcinoma on the background of active development of the tumor at the 45th day of the study. Pliss’ Lymphoma, and Lymphocytal Leukemia Р-388 models have been demonstrated to be optimal for the assessment of medicines’ aimed at VEGF elimination pharmacological activity.