Boosting Angiogenesis in Skin Mechanical Trauma Area by means of Neoskin Skin-Substitute Preparation

Various skin-replacements nowadays are widely used alongside other up-to-date methods for treatment of skin wounds for using this technique decreases the need for wound vascularization, increases the wound’s skin component, decreases or completely removes inhibitory factors, decreases inflammatory process thus contributing to quick and safe wound’s covering up ensuring tissue reparation flexibility. The goal of the study was to assess the influence of Neoskin skin-replacement upon angiogenesis and wound reparation intensity in experiment involving white rats. Skin wound was modeled by removing a 4,9 square mm skin flap by means of incision under general narcosis (Zoletil). The treatment of the wound included the application of innovation preparation Neoskin produced by “Trans-Technologies”, Russia upon the skin wound for 14 days. The preparation Neoskin is a three-layer matrix consisting of extracellular matrix proteins and mesenchimal cells. Angiogenesis markers concentrations were determined in blood plasma by means of immune-enzyme assay (VEGF, NO, tPA and PAI-1). Vascular network density in the wound surface zone was determined by means of histological method in slides stained with hemotoxillin-eosine with the aid of light-microscopy in 50 fields of vision. Skin mechanical trauma model not involving subjacent organs and tissues was accompanied by angiogenesis changes characteristic of wound healing process. Transitory reduction of microcirculatory network density in the area of the wound and increasing activity of basic proangiogenic factors necessary for reparation took place. The use of Neoskin in the therapy of skin lesions in rats has contributed to fast activation of angiogenesis and wound healing.