Ранний постнатальный стресс временной изоляции от матери у крысят способствует развитию у них тревожно-депрессивных симптомов во взрослом возрасте: ОРИГИНАЛЬНЫЕ СТАТЬИ

Сарнг Саналович Пюрвеев; Шуанет Абакаргаджиевна Ибрагимова; Анастасия Юрьевна Бодрова; Алефтина Алексеевна Кравцова; Татьяна Евгеньевна Лебедева; Анастасия Руслановна Авдеева; Аделина Михайловна Максюта; Анна Валентиновна Васильева; Родион Владимирович Кораблев; Никита Алексеевич Лужнов; Николай Сергоевич Деданишвили; Андрей Глебович Васильев; Михаил Сергеевич Некрасов; Андрей Андреевич Лебедев

doi:10.56871/

Early postnatal maternal temporary isolation stress in rats contributes to the development of anxiety-depressive symptoms in adulthood

ORIGINAL STUDIES

Sarng S. Pyurveev Saint Petersburg State Pediatric Medical University; Institute of Experimental Medicine https://orcid.org/0000-0002-4467-2269
Shuanet A. Ibragimova Institute of Experimental Medicine https://orcid.org/0009-0003-3709-7993
Anastasiya Yu. Bodrova Institute of Experimental Medicine https://orcid.org/0009-0001-9107-9871
Aleftina A. Kravtsova Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0002-0657-3390
Tatiana E. Lebedeva Saint Petersburg State Pediatric Medical University https://orcid.org/0009-0007-5494-6095
Anastasia R. Avdeeva Saint Petersburg State Pediatric Medical University https://orcid.org/0009-0004-3037-4545
Adelina M. Maksyuta Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0003-2043-8125
Anna V. Vasilieva Saint Petersburg State Pediatric Medical University https://orcid.org/0009-0008-2356-1552
Rodion V. Korablev Saint Petersburg State Pediatric Medical University https://orcid.org/0009-0004-5754-8437
Nikita A. Luzhnov Institute of Experimental Medicine; Samara State Medical University https://orcid.org/0009-0008-0628-4389
Nikolai S. Dedanishvili Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0001-6231-445X
Andrei G. Vasiliev Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0002-8539-7128
Mikhail S. Nekrasov Saint Petersburg State Pediatric Medical University https://orcid.org/0000-0002-9434-1433
Andrei A. Lebedev Institute of Experimental Medicine; Saint Petersburg University of Management Technologies and Economics https://orcid.org/0000-0003-0297-0425

Keywords:

early postnatal stress maternal temporary isolation during early ontogenesis anxiety depression ранний постнатальный стресс временная изоляция от матери в раннем онтогенезе тревожность депрессия

Abstract

BACKGROUND: Depressive disorders are becoming increasingly prevalent and represent a significant social issue with heavy economic implications.

AIM: To study the effects of maternal temporary isolation during early ontogenesis on the development of anxiety and depressive symptoms in adult rats.

MATERIALS AND METHODS: The study employed a maternal temporary isolation model as a form of early postnatal stress (from postnatal days 2 to 12). Two experimental groups were formed: a control group (n=20) and an “early maternal temporary isolation” group (n=20). On the 90th day of life, a behavioral test battery was used to assess the impact of early postnatal stress on the development of anxiety-depressive symptoms. The behavioral tests included the elevated plus maze, the Porsolt forced swim test, and the sucrose preference test.

RESULTS: Behavioral testing in the elevated plus maze revealed that rats exposed to early maternal temporary isolation showed reduced time spent in the open arms and increased time in the closed arms compared to the control group, indicating heightened anxiety levels. In the Porsolt test, the early isolation group demonstrated increased immobility time compared to the control group. In the sucrose preference test, the early isolation group exhibited reduced sucrose solution preference, indicative of anhedonia.

CONCLUSION: Stress exposure during early ontogenesis, a critical period for the development and maturation of brain structures responsible for psychoemotional behavior, can lead to their dysregulation and serves as a predictor for the development of anxiety-depressive symptoms in adult rats.

Author Biographies

Sarng S. Pyurveev, Saint Petersburg State Pediatric Medical University; Institute of Experimental Medicine

MD, Ph D, Assistant Professor, Department of Pathological Physiology with the Course of Immunopathology, Saint Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation; address: 2 Litovskaya st.,
Saint Petersburg, 194100, Russia; Research Associate, Department of Neuropharmacology, Institute of Experimental Medicine, Saint Petersburg, Russia

Shuanet A. Ibragimova, Institute of Experimental Medicine

Research Laboratory Assistant,
Department of Neuropharmacology named after S.V. Anichkov, Institute of Experimental Medicine, Saint Petersburg, Russia

Anastasiya Yu. Bodrova, Institute of Experimental Medicine

Research Laboratory Assistant, Department of Neuropharmacology named after S.V. Anichkov, Institute of Experimental Medicine, Saint Petersburg, Russia

Aleftina A. Kravtsova, Saint Petersburg State Pediatric Medical University

Ph D, Associate Professor, Department of Pathological Physiology with the Course of Immunopathology, Saint Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, Saint Petersburg, Russia

Tatiana E. Lebedeva, Saint Petersburg State Pediatric Medical University

Assistant Professor, Department of General and Medical Chemistry named after Prof. V.V. Khorunzhey, Saint Petersburg State Pediatric Medical University, Ministry of Health
of the Russian Federation, Saint Petersburg, Russia

Anastasia R. Avdeeva, Saint Petersburg State Pediatric Medical University

6th year Student of the Pediatric
Faculty, Saint Petersburg State Pediatric
Medical University, Ministry of Health
of the Russian Federation,
Saint Petersburg, Russia

Adelina M. Maksyuta, Saint Petersburg State Pediatric Medical University

6th year Student of the Pediatric Faculty, Saint Petersburg State Pediatric Medical University,
Ministry of Health of the Russian Federation,
Saint Petersburg, Russia

Anna V. Vasilieva, Saint Petersburg State Pediatric Medical University

MD, Ph D, Assistant Professor,
Department of Pathological Physiology with the Course of Immunopathology, Saint Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, Saint Petersburg, Russia

Rodion V. Korablev, Saint Petersburg State Pediatric Medical University

MD, Ph D, Assistant Professor,
Department of Pathological Physiology with the Course of Immunopathology, Saint Petersburg State Pediatric University, Ministry of Health of the Russian Federation, Saint Petersburg, Russia

Nikita A. Luzhnov, Institute of Experimental Medicine; Samara State Medical University

6th year Student of the
Pediatric Faculty of Samara State Medical University, Ministry of Health of the Russian Federation, Samara, Russia; laboratory research assistant, Department of Neuropharmacology named after S.V. Anichkov, Institute of Experimental Medicine, Saint Petersburg, Russia

Nikolai S. Dedanishvili, Saint Petersburg State Pediatric Medical University

6th year Student of the Pediatric Faculty, Saint Petersburg State Pediatric University, Ministry of Health of the Russian Federation, Saint Petersburg, Russia

Andrei G. Vasiliev, Saint Petersburg State Pediatric Medical University

MD, Ph D, Dr. Sci. (Medicine), Professor, Head of the Department of Pathological Physiology with a Course in Immunology, Saint Petersburg State Pediatric University, Ministry of Health of the Russian Federation, Saint Petersburg, Russia

Mikhail S. Nekrasov, Saint Petersburg State Pediatric Medical University

Postgraduate Student of the Department of Pharmacology with a Course of Clinical Pharmacology and Pharmacoeconomics, Saint Petersburg State Pediatric Medical University, Ministry of Health of the Russian Federation, Saint Petersburg, Russia

Andrei A. Lebedev, Institute of Experimental Medicine; Saint Petersburg University of Management Technologies and Economics

Ph D, Dr. Sci. (Pharmacology), Professor, Head of the Laboratory of General Pharmacology, Department of Neuropharmacology, Department of Neuropharmacology named after S.V. Anichkov, Institute of Experimental Medicine,
Saint Petersburg, Russia

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